ironjustice wrote:
- quote -

Make sure your turmeric, curry powder, and mustard are fresh when you
buy them, and (according to Perricone) keep them in the refrigerator.

--
Marshall Price of Miami
Known to Yahoo as d021317c

On Apr 7, 7:57*am, "ironjust...[at]aol.com" <ironjust...[at]aol.com> wrote:

Curcumin Suppresses IgE-Mediated Allergic Response and Mast Cell
Activation

Information from Industry
Assess clinically focused product information on Medscape.
Click Here for Product Infosites -- Information from Industry.



By Scott Baltic

NEW YORK (Reuters Health) Jun 03 - Curcumin, which gives curry its
yellow color, inhibits Syk kinase-dependent signaling events in mast
cells in mice and might therefore be useful in the treatment of mast
cell-related allergic diseases, according to a report by Korean and
U.S. researchers in the May issue of the Journal of Allergy and
Clinical Immunology.

Although curcumin, a natural constituent of the spice turmeric
(Curcuma longa), was previously known to have anti-allergic activity
in animal models, the current findings clarify the mechanism by which
it suppresses IgE-mediated allergic response. Syk, a tyrosine kinase,
plays an essential role in the IgE-dependent activation of mast cells.

Dr. Wahn Soo Choi of Konkuk University, Chungju, Korea, and colleagues
induced passive cutaneous anaphylaxis (PCA) in the ears of 4-week-old
male BALB/c mice. Curcumin administered orally 1 hour before injection
of antigen significantly suppressed the mast cell-dependent PCA
reaction in a dose-dependent manner.

Two cytokines, TNF-alpha and IL-4, are among those that are critical
to allergic inflammation. In studies using the RBL-2H3 tumor mast cell
line, the researchers found that curcumin significantly inhibited the
antigen-stimulated expression of mRNA for both cytokines, and the
secretion of both cytokines in the mast cells, in a dose-dependent
manner.

An examination of phosphorylation of and by Syk indicated that
although activation of upstream Src kinases and phosphorylation of Syk
itself were unaffected by curcumin, Syk-dependent downstream
phosphorylation of linkers and binders were significantly inhibited by
curcumin in a dose-dependent manner in both RBL-2H3 cells and bone
marrow-derived mast cells.

Based on these results, the researchers conclude, "The development of
mast cell inhibitors of Syk kinase might provide a reasonable approach
toward therapeutic intervention in allergic disorders."

"Various pharmaceutical formulations including curcumin, such as oral
tablets and topical ointments, may have some benefit for the treatment
of mast cell-mediated allergic diseases," Dr. Choi told Reuters
Health, "including allergic rhinitis, mast cell-dependent asthma,
eczema, and atopic dermatitis."

Dr. Choi added that curcumin has not been reported as showing any
significant toxicity in humans, but further study will be needed to
prove it has the same allergy-suppressing effect in humans.

J Allergy Clin Immunol 2008;121:1225-1231.

-----
Published online before print June 27, 2007,
10.1183/09031936.00019307

Eur Respir J 2007; 30:662-671
Copyright (c)ERS Journals Ltd 2007


Effect of choline chloride in allergen-induced mouse model of airway
inflammation
A. K. Mehta1,2, S. N. Gaur3, N. Arora1 and B. P. Singh1
1 Allergy and Immunology Section, Institute of Genomics and
Integrative Biology, and, 3 Dept of Respiratory Medicine, Vallabhbhai
Patel Chest Institute, University of Delhi, Delhi, and 2 Dept of
Biotechnology, University of Pune, Pune, India.


CORRESPONDENCE: B. P. Singh, Allergy and Immunology Section, Room No.
509, Institute of Genomics and Integrative Biology, Delhi University
Campus, Mall Road, Delhi-110007, India. Fax: 91 1127667471. E-mail:
singhbp1...[at]yahoo.com


Keywords: Airway inflammation, animal model, asthma, choline,
eosinophils


Received: February 16, 2007
Accepted June 20, 2007


The incidence of asthma has increased the world over, and current
therapies for the disease suffer from potential side-effects. This
has
created an opportunity to develop novel therapeutic approaches. Here,
the anti-inflammatory activity of choline was investigated in a mouse
model of allergic airway inflammation.


Choline (1 mg·kg-1) was administered via oral gavage or intranasally
before and after ovalbumin (OVA) challenge in sensitised mice. Airway
hyperresponsiveness (AHR) to methacholine was measured in the mice by
whole-body plethysmography. Type-2 T-helper cell cytokine and
leukotriene levels were estimated in bronchoalveolar lavage fluid
(BALF) and spleen culture supernatant by ELISA. Eosinophil peroxidase
activity was also determined in the BALF supernatant.


Choline treatment in sensitised mice before OVA challenge via oral/
intranasal routes significantly inhibited eosinophilic airway
inflammation and eosinophil peroxidase activity. It also reduced
immunoglobulin E and G1 production and inhibited the release of
type-2
T-helper cell cytokines and leukotrienes. However, the development of
AHR was prevented effectively by intranasal choline treatment. Most
importantly, choline treatment after OVA challenge by both routes
could reverse established asthmatic conditions in mice by inhibiting
AHR, eosinophilic airway inflammation and other inflammatory
parameters.


This study provides a new therapeutic approach for controlling as
well
as preventing asthma exacerbations.


---------------------------------------------------------------------------*------------


<<snip> >
Lecithin may therefore be the method of choice for
accelerating acetylcholine synthesis
<<snip> >


Lancet. 1977 Jul 9;2(8028):68-9. Related Articles, Links


Lecithin consumption raises serum-free-choline levels.


Wurtman RJ, Hirsch MJ, Growdon JH.


Consumption of choline by rats sequentially increases
serum-choline, brain-choline, and brain-acetylcholine
concentrations.
In man consumption of choline increases in levels in the
serum and cerebrospinal fluid; its administration is an
effective way of treating tardive dyskinesia.
We found that oral lecithin is considerably more effective
in raising human serum-choline levels than an equivalent
quantity of choline chloride.
30 minutes after ingestion of choline chloride (2-3 g free base),
serum-choline levels rose by 86% and returned to normal
values within 4 hours;
1 hour after lecithin ingestion, these levels rose by 265% and
remained significantly raised for 12 hours.
Lecithin may therefore be the method of choice for
accelerating acetylcholine synthesis by increasing the availability
of choline, its precursor in the blood.


PMID: 69151 [PubMed - indexed for MEDLINE]


---------------------------------------------------------------------------*------------
"Acetylcholine release can be enhanced by consumption of certain
foods"


Science 12 August 1983:
Vol. 221. no. 4611, pp. 614 - 620
DOI: 10.1126/science.6867732


Articles


Choline and cholinergic neurons
JK Blusztajn and RJ Wurtman


Mammalian neurons can synthesize choline by methylating
phosphatidylethanolamine and hydrolyzing the resulting
phosphatidylcholine. This process is stimulated by catecholamines.
The
phosphatidylethanolamine is synthesized in part from
phosphatidylserine; hence the amino acids methionine (acting after
conversion to S-adenosylmethionine) and serine can be the ultimate
precursors of choline. Brain choline concentrations are generally
higher than plasma concentrations, but depend on plasma
concentrations
because of the kinetic characteristics of the blood-brain-barrier
transport system. When cholinergic neurons are activated,
acetylcholine release can be enhanced by treatments that increase
plasma choline (for example, consumption of certain foods).
Science, Vol 221, Issue 4611, 614-620
Copyright (c) 1983 by American Association for the Advancement of
Science


---------------------------------------------------------------------------*-----


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/a3cc3


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk





- quote -

On Apr 6, 3:55*pm, Marshall Price <d0213...[at]yahoo.com> wrote:What are
you trying to say?<<

If you look closely it is pretty clear what the thread was about ..

Look for the question mark .. data mine .. is there a question
mark .. ?

Yes .. there IS ..
<<snip> >
So what **difference** would there BE in the processing of the
hops ..
"hop water extraction (HWE) has anti-allergic effects " .. and ..
"this activity was not observed for the hot water extract from the
hops." .. ?


'Something' is .. missing .. and that something is the inhibitor of
the allergic response .. LOST .. to the process of extraction .. to
'heat' .. ?
<<snip> >

On Apr 6, 3:55 pm, Marshall Price <d0213...[at]yahoo.com> wrote:That we
should all travel back 25 years in time to read old "Science"
articles about cholinergic neurons because...what? <<

It is called .. data mining .. in this case it is health research
analysis ..

On Apr 6, 3:55 pm, Marshall Price <d0213...[at]yahoo.com> wrote: Because
they mention
the word "choline"?! So what? <<

Now there is where the thinking part comes in .. Marshall ..

Can you think .. ?

Do you have the .. **capacity** to **store** .. information .. IN your
brain .. OR thanks to the advent of the computer .. do you have the
ABILITY to decipher / correlate / hypothesise .. health research
studies ..

Those are a couple of the prerequisits to UNDERSTANDING .. anything ..
anyone .. anywhere .. IN .. health research .. threads ..

This was an article about .. choline and its effects on the asthma
response ..

The second group / post was in reference to .. ? .. to .. ?

Asthma .. and how its treated effectively with hops .. PART of the
hops ..

Sooo .. thats where the .. ? .. question mark is .. relevant ..

That is where you missed the **gist** OF the thread ..

It possibly would invoke .. thinking ..

It also mentioned lipids .. but I'm not REALLY sure what they said ..


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/a3cc3


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk



- quote -

On Apr 6, 3:55*pm, Marshall Price <d0213...[at]yahoo.com> wrote:This is
really not very entertaining, you know. <<

"It hurts when I think" .. ?


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/a3cc3


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk



- quote -

ironjustice[at]aol.com wrote:
- quote -

What are you trying to say?

That we should all travel back 25 years in time to read old "Science"
articles about cholinergic neurons because...what? Because they mention
the word "choline"?!

So what?

This is really not very entertaining, you know.

--
Marshall Price of Miami
Known to Yahoo as d021317c

On Apr 4, 9:30*am, "ironjust...[at]aol.com" <ironjust...[at]aol.com> wrote:
choline <<

Allergic Response Tied to Lipid Molecules in Cell Membrane

7 March 2008--A team of Penn State University researchers is the
first
to demonstrate that lipid molecules in cell membranes participate in
mammals' reactions to allergens in a living cell. The finding will
help scientists better understand how allergy symptoms are triggered,
and could contribute to the creation of improved drugs to treat them.
The work will be reported in the 14 March issue of the Journal of
Biological Chemistry.


The team studied clusters of cholesterol-rich lipid molecules that
they believe serve as platforms for the receptors that receive
antibodies, the proteins that protect the body from allergens. In
this case, the team examined IgE antibodies, which upon binding to
their receptors initiate a cell's release of histamine--the substance
that causes the unpleasant, but beneficial, mucous production,
congestion, and itchiness associated with allergies. "This research
is basically the molecular foundation for why many people sneeze in
the spring," said Ahmed Heikal, an associate professor in the
Department of Bioengineering and a leader of the project.


While the idea that lipid clusters--also known as lipid domains--are
involved in the allergic response is not new, the Penn State team is
the first to document this connection in a living cell under
physiological conditions. "No one has observed the domains in action
because they are too small and too transient--held together by very
weak molecular interactions--to be viewed with a light microscope,"
said Erin Sheets, a Penn State assistant professor of chemistry who
also is a leader of the project. "To overcome this challenge," added
Heikal, "we used a combination of imaging and spectroscopy techniques
that we are developing in our laboratories.


In their experiment, the researchers first labeled the cell membrane
and IgE antibodies with two different fluorescent tags. Next, they
introduced an allergen and watched as it bound to receptors on the
cell membrane, thus initiating an allergic response.


But to demonstrate that this activity was taking place within the
lipid domain, the researchers had to take advantage of a property of
fluorescence, called fluorescence lifetime, in which molecules are
excited with very short laser pulses. The length of time a molecule
remains in its excited state before emitting a photon--the
fluorescence lifetime--provides unique information about the
fluorescently-labeled molecule's environment and its chemical
structure. For example, a particular molecule might relax to its
lowest-energy state quickly or slowly depending on whether it is
exposed to a solvent.

Nanostructural changes in the plasma membrane occur upon antigen
stimulation.
"We previously showed that our fluorescently-labeled membrane probe
has a longer lifetime within a cholesterol-rich lipid domain," said
Sheets. "Here we show that changes in this lifetime follow the
changes that occur during the first steps in the allergic response
process. Our results also show that lipid domains in the cell
membrane associate with IgE antibodies and their receptors in the
initial stages of an allergic reaction."


In the future, Sheets and Heikal plan to apply the team's discoveries
to a project involving aging. During the aging process, T cells,
which protect the body from foreign substances like viruses and
cancer
cells, can lose their ability to signal effectively. Sheets and
Heikal plan to use these fluorescence-lifetime imaging tools to
examine the structure and integrity of T-cell membranes with a goal
of
determining why they lose their knack for signalling and how this
problem can be corrected.


"We want to compare the effectiveness of signaling in young T cells,
which clear out debris quickly, to old T cells, which are not as
efficient," said Sheets. "I think it will be a pretty cool
application
of our technique."


Other Penn State scientists who contributed to this research include
Angel Davey and Keith Krise, both Ph.D. students in the Department of
Chemistry. The work was funded by Penn State, the National Science
Foundation, the Commonwealth of Pennsylvania, the American Chemical
Society, and the National Institutes of Health.
Erin Sheets: (+1) 814-863-0044, ed...[at]psu.edu
Ahmed Heikal: (+1) 814-865-8093, aa...[at]psu.edu
Barbara Kennedy (PIO): (+1) 814-863-4682, scie...[at]psu.edu
--------------------------
So what **difference** would there BE in the processing of the
hops ..
"hop water extraction (HWE) has anti-allergic effects " .. and ..
"this activity was not observed for the hot water extract from the
hops." .. ?


'Something' is .. missing .. and that something is the inhibitor of
the allergic response .. LOST .. to the process of extraction .. to
'heat' .. ?


Presentation Number: 191-18
Abstract Division: Nutraceuticals and Functional Foods
Presentation Start/End Time: Tuesday, Jul 31, 2007, 2:00 PM - 5:30 PM
Author Information: Yoshihisa Wakita, Frontier Laboratories of Value
Creation, Sapporo breweries LTD., Yaizu, Japan; Yoshihiro Takata,
Frontier Laboratories of Value Creation, Sapporo breweries LTD.,
Yaizu, Japan; Syuuichi Segawa, Frontier Laboratories of Value
Creation, Sapporo breweries LTD., Yaizu, Japan; Yasukazu Nakakita,
Frontier Laboratories of Value Creation, Sapporo breweries LTD.,
Yaizu, Japan; Hirotaka Kaneda, Frontier Laboratories of Value
Creation, Sapporo breweries LTD., Yaizu, Japan; Junji Watari,
Frontier
Laboratories of Value Creation, Sapporo breweries LTD., Yaizu, Japan;
Tatsuko Enomoto, Enomoto ENT Clinic, Wakayama, Japan; Tadao Enomoto,
Japanese Red Cross Society, Wakayama Medical Center, Wakayama, Japan
Abstract: Hops (Humulus lupulus L.) are used in the brewing of beer
and are available throughout the world. During evaluation of the
physiological functions of hops, it was shown that hop water
extraction (HWE) has anti-allergic effects in vitro and in vivo. HWE
suppressed histamine release from the human basophilic KU812 cells;
however, this activity was not observed for the hot water extract
from
the hops. An oral dose of 500 mg/kg of HWE significantly inhibited
vascular permeability induced by the intradermal injection of
compound
48/80 in ICR mice. This study also tested the effect of HWE in the
treatment of seasonal allergic rhinitis during the major season of
this allergy in Japan. In a 12-week randomized, double-blind,
placebo-
controlled clinical trial, patients with seasonal allergic rhinitis
were given 100mg of HWE (n=20) or placebo (n=19) per day. The three
nasal symptoms (sneezing, runny nose and stuffiness) and one non-
nasal
symptom (hindrance to daily life) were scored using a 5-point scale
daily: 0, absent; 1, mild; 2, moderate; 3, severe; and 4, very
severe.
The total symptom scores were calculated from the sum of the above 4
symptom scores per week and changes of those between the first week
and each week were calculated. During the trial period, the elevation
of symptom scores was observed as the dispersion of pollen in the
trial area. However, after 10 weeks, the patients of the HWE group
showed significantly lower changes of total symptom scores from the
first week compared to the placebo group (P<0.05). Thus, HWE is an
effective foodstuff for the improvement of the quality of life for
patients with seasonal allergic rhinitis, and would be easily applied
to beverages.


-------------------------
J Agric Food Chem. 2007 Oct 31;55(22):9054-8. Epub 2007 Oct 10. Links
Effects of phytic Acid on peanut allergens and allergenic properties
of extracts.
Chung SY, Champagne ET.
sych...[at]srrc.ars.usda.gov.


Phytic acid would form soluble and insoluble complexes with proteins.
Our objective was to determine if phytic acid forms insoluble
complexes with major peanut allergens, and if such reaction results
in
a peanut extract with a lower level of soluble allergens and
allergenic property. Extracts from raw and roasted peanuts were
treated with and without phytic acid at various pH values and then
analyzed by SDS-PAGE and a competitive inhibition ELISA (ciELISA).
The
ciELISA measured IgE binding using a pooled serum from peanut-
allergic
individuals. Results showed that phytic acid formed complexes with
the
major peanut allergens (Ara h 1 and Ara h 2), which were insoluble in
acidic and neutral conditions. Succinylation of the allergens
inhibited complex formation, indicating that lysine residues were
involved. A 6-fold reduction in IgE binding or allergenic potency of
the extract was observed after treatment with phytic acid. It was
concluded that phytic acid formed insoluble complexes with the major
peanut allergens, and resulted in a peanut extract with reduced
allergenic potency. Application of phytic acid to a peanut butter
slurry presented a similar result, indicating that phytic acid may
find use in the development of hypoallergenic peanut-based products.


PMID: 17927201 [PubMed - in process]


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/a3cc3


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk





- quote -

Published online before print June 27, 2007, 10.1183/09031936.00019307

Eur Respir J 2007; 30:662-671
Copyright (c)ERS Journals Ltd 2007

Effect of choline chloride in allergen-induced mouse model of airway
inflammation
A. K. Mehta1,2, S. N. Gaur3, N. Arora1 and B. P. Singh1
1 Allergy and Immunology Section, Institute of Genomics and
Integrative Biology, and, 3 Dept of Respiratory Medicine, Vallabhbhai
Patel Chest Institute, University of Delhi, Delhi, and 2 Dept of
Biotechnology, University of Pune, Pune, India.

CORRESPONDENCE: B. P. Singh, Allergy and Immunology Section, Room No.
509, Institute of Genomics and Integrative Biology, Delhi University
Campus, Mall Road, Delhi-110007, India. Fax: 91 1127667471. E-mail:
singhbp1951[at]yahoo.com

Keywords: Airway inflammation, animal model, asthma, choline,
eosinophils

Received: February 16, 2007
Accepted June 20, 2007

The incidence of asthma has increased the world over, and current
therapies for the disease suffer from potential side-effects. This has
created an opportunity to develop novel therapeutic approaches. Here,
the anti-inflammatory activity of choline was investigated in a mouse
model of allergic airway inflammation.

Choline (1 mg·kg-1) was administered via oral gavage or intranasally
before and after ovalbumin (OVA) challenge in sensitised mice. Airway
hyperresponsiveness (AHR) to methacholine was measured in the mice by
whole-body plethysmography. Type-2 T-helper cell cytokine and
leukotriene levels were estimated in bronchoalveolar lavage fluid
(BALF) and spleen culture supernatant by ELISA. Eosinophil peroxidase
activity was also determined in the BALF supernatant.

Choline treatment in sensitised mice before OVA challenge via oral/
intranasal routes significantly inhibited eosinophilic airway
inflammation and eosinophil peroxidase activity. It also reduced
immunoglobulin E and G1 production and inhibited the release of type-2
T-helper cell cytokines and leukotrienes. However, the development of
AHR was prevented effectively by intranasal choline treatment. Most
importantly, choline treatment after OVA challenge by both routes
could reverse established asthmatic conditions in mice by inhibiting
AHR, eosinophilic airway inflammation and other inflammatory
parameters.

This study provides a new therapeutic approach for controlling as well
as preventing asthma exacerbations.

-------------------------------------------------------

<<snip> >
Lecithin may therefore be the method of choice for
accelerating acetylcholine synthesis
<<snip> >


Lancet. 1977 Jul 9;2(8028):68-9. Related Articles, Links


Lecithin consumption raises serum-free-choline levels.


Wurtman RJ, Hirsch MJ, Growdon JH.


Consumption of choline by rats sequentially increases
serum-choline, brain-choline, and brain-acetylcholine
concentrations.
In man consumption of choline increases in levels in the
serum and cerebrospinal fluid; its administration is an
effective way of treating tardive dyskinesia.
We found that oral lecithin is considerably more effective
in raising human serum-choline levels than an equivalent
quantity of choline chloride.
30 minutes after ingestion of choline chloride (2-3 g free base),
serum-choline levels rose by 86% and returned to normal
values within 4 hours;
1 hour after lecithin ingestion, these levels rose by 265% and
remained significantly raised for 12 hours.
Lecithin may therefore be the method of choice for
accelerating acetylcholine synthesis by increasing the availability
of choline, its precursor in the blood.


PMID: 69151 [PubMed - indexed for MEDLINE]


------------------------------------------------------- "Acetylcholine release can be enhanced by consumption of certain
foods"

Science 12 August 1983:
Vol. 221. no. 4611, pp. 614 - 620
DOI: 10.1126/science.6867732

Articles

Choline and cholinergic neurons
JK Blusztajn and RJ Wurtman

Mammalian neurons can synthesize choline by methylating
phosphatidylethanolamine and hydrolyzing the resulting
phosphatidylcholine. This process is stimulated by catecholamines. The
phosphatidylethanolamine is synthesized in part from
phosphatidylserine; hence the amino acids methionine (acting after
conversion to S-adenosylmethionine) and serine can be the ultimate
precursors of choline. Brain choline concentrations are generally
higher than plasma concentrations, but depend on plasma concentrations
because of the kinetic characteristics of the blood-brain-barrier
transport system. When cholinergic neurons are activated,
acetylcholine release can be enhanced by treatments that increase
plasma choline (for example, consumption of certain foods).
Science, Vol 221, Issue 4611, 614-620
Copyright (c) 1983 by American Association for the Advancement of
Science

------------------------------------------------
Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/a3cc3


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk